Enzymes of Interest

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Families of Interest

  • glycoside hydrolases
    • endo-acting cellulases
    • exo-acting glucosidases
    • endo-glucanases
  • ligninases
    • lignin peroxidases
    • laccases - copper-containing oxidase enzymes
  • pectinases and pectinesterases
    • pectin lyases
    • rhamnogalacturonan lyase
    • pectin methylesterase
    • polygalacturonase
  • hemicellulase enzymes
    • endo-1,4-beta-xylanases
    • feruloyl esterase
    • α-glucuronidases
    • arabinofuranosidases
    • acetylxylan esterases
  • oxidoreductases
    • cellobiose dehydrogenase
    • Manganese peroxidase
    • versatile peroxidase

Enzyme Substrates

  • Cellulose - a polysaccharide consisting of a linear chain of several hundred to over ten thousand β(1→4) linked D-glucose units. Cellulose is the structural component of the primary cell wall of green plants (~33% of all plant matter).
  • Lignin - a cross-linked racemic macromolecule with molecular masses in excess of 10,000 u. It is relatively hydrophobic and aromatic in nature. There are three monolignol monomers, methoxylated to various degrees: p-coumaryl alcohol, coniferyl alcohol, and sinapyl alcohol. Lignin is an integral part of the secondary cell walls of plants and constitutes from a quarter to a third of the dry mass of wood.
  • Pectin - a family of complex polysaccharides that contain 1,4-linked α-D-galactosyluronic acid residues. Pectins are present in most primary cell walls and particularly abundant in the non-woody parts of terrestrial plants.
  • Hemicellulose - can be any of several heteropolymers (matrix polysaccharides) with a random, amorphous structure. It consists of 500-3000 sugar units (including xylose, mannose, galactose, rhamnose, and arabinose) with a branched structure. Hemicellulose is present in almost all plant cell walls.
  • Cellobiose - is a disaccharide of two glucose molecules linked in a β(1→4) bond. It can be obtained by enzymatic or acidic hydrolysis of cellulose and cellulose rich materials such as cotton, jute, or paper.
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